المستخلص
The study samples were collected from Baghdad Teaching Hospital / The Joints Consolatory in Baghdad Gouvernante during the period Dec 15th 2021 to March 30th 2022. This study aimed to uncover the spread of Cytomegalovirus in the rheumatoid arthritis patients, study the immunological variance of IL-12 and IL-35, measuring Resistin protein levels, and the study of the genetic variance for the Single Nucleotide Polymorphism ( IL-12 rs3212227, IL -12 rs2243115, IL-35 rs4740, IL-35 rs2243131). The study included 102 samples from both genders of both genders and different age categories (18-68) years old, the samples were in to two groups: the first group consisted of the 72 rheumatoid arthritis patients and the second group is the control group (healthy subjects) consisted of 30 samples. Blood samples were collected from rheumatoid arthritis patients attending The Joints Consolatory in Baghdad, immunological tests for Anti-CMV IgG, Anti-CMV IgM, Resistin protein and the cytokines IL-12 and IL-35 with ELISA technology. The Single Nucleotide Polymorphism was studied using Taq Man Real Time technology.
The study results showed there were no significant differences between study groups and age (p>0.05), also the study showed that females are more susceptive to rheumatoid arthritis than males with no significant difference (p>0.05). The study also revealed elevated Anti-CMV IgM and Anti-CMV IgG in rheumatoid arthritis patients compared to control group with significant difference (p<0.05).
The study results also referred to elevated IL-12, IL-35, and Resistin protein levels in rheumatoid arthritis patients compared to control group with no significant difference (p>0.05).
The study of IL-12 rs3212227 revealed that the genomic pattern GG and allele G are more frequent in rheumatoid arthritis patients compared to control group and the pattern TG and allele T are less frequent in rheumatoid arthritis patients compared to control group. The odds ratio refers that the allele G is considered a risk factor and the allele T is a protection factor. The study results revealed there was no significant correlation between IL-12 and IL-12 rs3212227 (p>0.05). the study results also referred there is a significant correlation between IL-12 rs3212227 and Cytomegalovirus (p<0.05).
The study results of IL-12 rs2243115 showed that the TT genetic pattern and the allele T are more frequent in rheumatoid arthritis patients compared to control group. While the genetic pattern GG and the allele G are less frequent in rheumatoid arthritis patient compared to control group. The odds value refers that the allele T is considered a risk factor and the allele G is considered a protection factor. Also, the study results showed that there is no significant correlation between IL-12 and IL-12 rs2243115 levels (p>0.05). The study results also showed there is a statistical correlation between Cytomegalovirus and IL-12 rs2243115 (p<0.05).
The study results for IL-35 rs4740 that the genetic patterns GA and AA are more frequent in rheumatoid arthritis patients compared to control group. And the genetic pattern GG was less frequent in rheumatoid arthritis patient compared to control group. The allele A was more frequent and allele G was less frequent. The odds value refers that the allele A is considered as a risk factor and allele G is considered as a protection factor. Also noticed a significant correlation (p<0.05) between IL-35 and the patterns GG and AA. The current study results showed there is a significant correlation between Anti-CMV IgM and the patterns AA and IL-35 rs4740 GA in rheumatoid arthritis patients (p<0.05). also, the study results showed a significant correlation between Anti-CMV IgG and IL-35 rs4740 in rheumatoid patients (p<0.05).
Our study results showed that the genetic pattern CC, AC IL-35 rs2243131 is more frequent in rheumatoid arthritis patients compared to control group. The genetic pattern AA IL-35 rs2243131 is less frequent in rheumatoid arthritis patients compared to control group. The allele C was more frequent and allele A was less frequent. The odds value refers that the allele C is considered as a risk factor and allele A is considered as a protection factor. The study results showed a significant correlation between Anti-CMV IgM and the pattern IL-35 AC rs2243131 (p<0.05). also has been noticed there is a significant correlation between Anti-CMV IgG and IL-35 rs2243131 (p<0.05).
