Abstract

In this research, the synthesized compounds were divided into two parts:

The first part included new azo ligands  )L₁,L₂) have been produced all resting on the Luminol as a backbone of the prepared ligands having the following structures.

L₁=

L₂=

Scheme (1): General scheme of the synthesized compounds

        Produced azo ligands have been characterized by (FT-IR ,¹H-¹³C NMR –NMR, UV-Vis and LC-Mass ) spectra and (C.H.N.O) analysis.

The second part  of this project  refers to the synthesis of metal chelates Co^II , Ni^II, Cu^II,Pd ^II and Pt^IV with two prepared azo ligands . All production was performed after fixing the ideal pH and molar concentration that obeyed Lambert –Beers law in the studies pH rates. According to the molar ratio methods- the structure of these metal chelates was deduced depending on the spectroscopic studies of the complex solution of the above ions. However, ratio of 1:2 M:L for Co^II, Ni^II ,Cu^II,Pt ^IV ions were obtained . The mole ratio of Pd ^II complex was 1:1 M:L . FT-IR, UV-Vis spectra, conductivity, magnetic susceptibility and melting points measurements were used to characterize  prepared complexes.

the Flame atomic absorption technique has been measured to calculate the percentage of the metals in the complexes. (C.H.N.O) analysis were also found, and the results are in agreement with the calculated values.

All the complexes are quite stable and could be stored for months without any appreciable change. Depending on the results obtained by elemental and spectral  analysis , an octahedral  structure  is suggested for Co^II ,Ni^II , Cu^II , Pt ^IV complexes .and square Planar for the  Pd^II complex .

In addition the dyeing performance of the produced compound has been assessed on cotton fabric , the dyes were tested for light and detergent fastness .

Finally evaluation of biological activities for ligands L₁ and L₂ and its complexes on Mitotic Index(chromosome) in lymphocytes of humans . the compounds were compared with the colchicine . Show the  L₁ pd complex was the highest inhibition rate was recorded as 3.97d ±  0.02 at aconcentration of 100 mg\ml than to the L₁  and L₁ pt_. while the ligand L₂ recorded the highest inhibition rate at 3.78 d±0.02 at aconcentration of 100 mg\ml than to the its complexes .