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رسالة ماجستير / ياسين محسن


Anti-cancer and acute toxicity of a new Indole shiff bass   (2IP) was tested for the antioxidant ability, toxicity on WRL 68 cell line and anti-tumor activity on MCF-7 and CAM-1  breast cell line. The result shows that 2IP has antioxidant ability as IC50 (53µg/mL) compared with ascorbic (28 µg/mL). 2IP has less toxic effect on WRL 68 cell line in dose dependent manner for 24,48 and 72 hours, While 2IP has anti-tumor effect on MCF-7 and CAM-1 cell line in dose dependent manner for 24,48 and 72 hours. The antitumor effect is by enhancement  apoptosis, the alteration in nuclear morphology of MCF-7 cell line was examined by using Hoechest staining after treatment of MCF-7 cells with different doses (5,10,15) µg/mL . The intensity of the nucleus that directly related to the apoptosis chromatin changes occurred. The increase in the cells membrane permeability was observed. The result  show decreasing in the nucleus intensity and increasing in cell permeability with the increasing   concentration of 2IP compound treated  cells.

MMP is distrusted in the apoptosis by forming of permeability, this ability of 2IP on the MMP of MCF-7 cell was studied by using of mitochondria specific dye. The MMP in the MCF-7 cell treated with 2IP showed a significant (P < 0.05) reduction by decrease in the intensity of fluorescent compared with the control. The effect of 2IP in the treated MCF-7 cell for cytochrome c was detected, the result showed that 2IP activated release cytochrome c is significant (P < 0.05) higher than control.

          The result of this study   approved that 2IP disturb the DNA content of MCF-7 cells in the cell cycle phase (G1, G2, and M) after treatment. 2IP break down the cell cycle progress in G1 phase (P <0.05) compared with the control. The result displayed that there is a significant G1 phase arrest in a concentration dependent manner in MCF-7 cells , whereas the cells in both G2 and M phase was reduced with an increase in the treatment. In conclusion, the indole shift synthetic compound 2IP has an antioxidant and anti-tumor ability that could be used as a new anti-cancer drug.

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